The relationship between airtightness and ventilation in new UK dwellings

The ATTMA airtightness testing competent persons scheme collects pressure test data and metadata from the majority of new build dwellings in the UK. This article uses the dataset to investigate the importance of the ventilation strategy in airtightness design and construction. Design and measured airtightness were tested for association with declared ventilation strategy. It was found that ventilation strategy makes a statistically significant difference to airtightness, however this difference is too small to be practically relevant. Properties with mechanical ventilation and heat recovery (MVHR) were shown to have a mean designed air permeability only 0.46 m3/m2h lower than naturally ventilated dwellings. 73% of homes with MVHR have design airtightness greater than or equal to 5 m3/m2h and 17% of naturally ventilated dwellings have design airtightness less than 5 m3/m2h. We discuss how current design is not maximising the CO2, cost and air quality benefit of each ventilation strategy. A new approach to regulatory compliance is proposed which explicitly links the designed airtightness and chosen ventilation system. It is suggested that compliance could then be achieved using a set of airtightness ranges linked to appropriate ventilation strategies. This could be expected to result in reduced energy consumption and carbon emissions for new build homes compared to the current approach, and would also potentially lead to better outcomes for occupants in terms of indoor air quality

A multi-gene phylogeny for species of Mycosphaerella occurring on Eucalyptus leaves

Species of the ascomycete genus Mycosphaerella are regarded as some of the most destructive leaf pathogens of a large number of economically important crop plants. Amongst these, approximately 60 Mycosphaerella spp. have been identified from various Eucalyptus spp. where they cause leaf diseases collectively known as Mycosphaerella Leaf Disease (MLD). Species concepts for this group of fungi remain confused, and hence their species identification is notoriously difficult. Thus, the introduction of DNA sequence comparisons has become the definitive characteristic used to distinguish species of Mycosphaerella. Sequences of the Internal Transcribed Spacer (ITS) region of the ribosomal RNA operon have most commonly been used to consider species boundaries in Mycosphaerella. However, sequences for this gene region do not always provide sufficient resolution for cryptic taxa. The aim of this study was, therefore, to use DNA sequences for three loci, ITS, Elongation factor 1-alpha (EF-1α) and Actin (ACT) to reconsider species boundaries for Mycosphaerella spp. from Eucalyptus. A further aim was to study the anamorph concepts and resolve the deeper nodes of Mycosphaerella, for which part of the Large Subunit (LSU) of the nuclear rRNA operon was sequenced. The ITS and EF-1α gene regions were found to be useful, but the ACT gene region did not provide species-level resolution in Mycosphaerella. A phylogeny of the combined DNA datasets showed that species of Mycosphaerella from Eucalyptus cluster in two distinct groups, which might ultimately represent discrete genera

A simple method for detection of mitochondrial DNA polymorphisms

Polymorphisms were identified in mtDNA of Heterobasidion annosum by digesting total genomic DNA with HaeIII, CfoI, or MspI, which recognize the restriction sites GGCC, GCGC, and CCGG, respectively. Most DNA was digested to fragments of less than 2 kb, while AT-rich fragments of 1.7 to 17 kb stood out as bands of uniform intensity after ethidium bromide staining. These fragments hybridized to mtDNA probes and were inherited in a uniparental fashion

Metabolic profile of long-distance migratory flight and stopover in a shorebird

Migrating birds often complete long non-stop flights during which body energy stores exclusively support energetic demands. The metabolic correlates of such long-distance travel in free-living migrants are as yet poorly studied. Bar-tailed godwits, Limosa lapponica taymyrensis, undertake a 4500 km flight to their single spring stopover site and thus provide an excellent model in which to determine the energy fuels associated with endurance travel. To this end, we evaluated plasma concentrations of six key metabolites in arriving godwits caught immediately upon landing near their stopover site. Initial metabolite levels were compared with levels after 5 h of inactive rest to determine how flight per se affects energy metabolism. Birds refuelling on the stopover site were also examined. Arriving godwits displayed elevated plasma free fatty acids, glycerol and butyrate, confirming the importance of lipid fuel in the support of extended migratory activity. Furthermore, elevated plasma triglycerides in these birds suggest that fatty acid provisioning is facilitated through hepatic synthesis and release of neutral lipids, as previously hypothesized for small migrants with high mass-specific metabolic rates. Finally, elevations in plasma uric acid suggest that protein breakdown contributes to the support of long-distance movement, to possibly maintain citric acid cycle intermediates, gluconeogenesis and/or water balance

Tree invasions and biosecurity: eco-evolutionary dynamics of hitchhiking fungi

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Does compulsory airtightness testing result in airtight buildings or creative ways of passing the test?

The ATTMA airtightness testing competent person scheme collects pressure test data from the majority of new build dwellings in the UK. A dataset of around 150,000 dwellings is available for analysis and shows surprising results. Measured airtightness for the first recorded test per dwelling is disproportionately concentrated at sharp peaks related to design targets. This is contrary to the skewed normal distribution expected from a process of building, testing, remedial works to seal the primary air barrier and retesting, and indicates that short term measures are being undertaken to pass the test that may not ultimately result in airtight buildings. We reflect on the purpose of airtightness targets and suggest ways in which the regulatory environment could encourage good design and consistent quality of construction

‘Hitting the target and missing the point’: Analysis of air permeability data for new UK dwellings and what it reveals about the testing procedure

Airtightness testing is widely undertaken to assess the as-built performance of dwellings, in support of achieving energy and ventilation strategies. Mandatory schemes operate in some countries, such as the UK, to ensure that dwellings are built in accordance with their design air permeability. However, testing is only useful if the results give a true picture of the airtightness of the building. Previous literature has investigated factors which could influence airtightness test results but has not questioned data quality, despite the pressure on builders to achieve design targets. This paper presents air permeability results from the largest UK dataset, comprising 144,024 dwellings tested under the Air Tightness Testing and Measurement Association (ATTMA) scheme. The data show an unexpected distribution of test results with narrow peaks just within test targets. Such results were not expected theoretically but do reflect findings in other fields where performance-based targets are in place. Such a close match between design and tested airtightness may be achieved by remedial works taking place during the test rather than afterwards. Recommendations are made with respect to quality assurance systems, design guidance and on-site sealing practices to increase the likelihood of long-term airtight buildings being constructed first time

Bark beetle population dynamics in the Anthropocene: Challenges and solutions

Tree-killing bark beetles are the most economically important insects in conifer forests worldwide. However, despite N200 years of research, the drivers of population eruptions and crashes are still not fully understood and the existing knowledge is thus insufficient to face the challenges posed by the Anthropocene. We critically analyze potential biotic and abiotic drivers of population dynamics of an exemplary species, the European spruce bark beetle (ESBB) (Ips typographus) and present a multivariate approach that integrates the many drivers governing this bark beetle system. We call for hypothesis-driven, large-scale collaborative research efforts to improve our understanding of the population dynamics of this and other bark beetle pests. Our approach can serve as a blueprint for tackling other eruptive forest insects

A chromosome-level genome assembly of a free-living white-crowned sparrow (Zonotrichia leucophrys gambelii)

The white-crowned sparrow, Zonotrichia leucophrys, is a passerine bird with a wide distribution and it is extensively adapted to environmental changes. It has historically acted as a model species in studies on avian ecology, physiology and behaviour. Here, we present a high-quality chromosome-level genome of Zonotrichia leucophrys using PacBio and OmniC sequencing data. Gene models were constructed by combining RNA-seq and Iso-seq data from liver, hypothalamus, and ovary. In total a 1,123,996,003 bp genome was generated, including 31 chromosomes assembled in complete scaffolds along with other, unplaced scaffolds. This high-quality genome assembly offers an important genomic resource for the research community using the white-crowned sparrow as a model for understanding avian genome biology and development, and provides a genomic basis for future studies, both fundamental and applied.</p